plasmid DNA DNA DNA filiation Introduction Chitobiase, from Vibrio harveyi, is a membrane bound lipoprotein bear on in the degradation of chitin. Chitobiase is uniform to and whitethorn share a common ancestry to the a-chain of serviceman b-hexos-aminidase. Chitobiase is encoded by chb. In this experiment, a bar routine for lying-in enzymes Eco R1, Pst1 and posterior III exploitation Southern hybridization and restriction abstract of pRSG 192. pRSG 192 is a recombinant plasmid derived from the chb gene and pUC 19, a 2.7kb engineered plasmid which encodes for ampicillin resistance, a impute of the lac operon and a multiple copy land . The chb gene exists as a 3.
6 kb precede in the mutiple cloning share of pUC 19. The major goals of Experiment mavin pass on be to isolate pRSG 192 from an overnight burnish of E. coli, amplify a portion of the chb gene using PCR, and to map restriction sites deep push down the chb gene using restriction analysis and Southern hybridization. Methods plasmid DNA Isolatio...If you want to get a full essay, order it on our website:
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